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Welcome to the interactive web schedule for the 2019 Midwest Fish & Wildlife Conference! The schedule is subject to change (as of December 11, 2018). Please check back for updates. To return to the main Conference website, go to: www.midwestfw.org.

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Tuesday, January 29 • 6:00pm - 9:00pm
(P88) Preliminary Genetic Evidence of Predation on an Imperiled Minnow from an Intentionally Introduced Apex Predator in a Western Ohio Stream

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AUTHORS. Kenneth J. Oswald, Ohio Northern University

ABSTRACT. Intentional introductions of non-native fishes into inland waters has been a cornerstone of North American freshwater fisheries management for well over a century. These introductions are often done to satisfy intense demand from recreational angling. Sport fishers tend to value large-bodied species that display aggressive behaviors, two life history characteristics of apex predators. Tonguetied minnow (Exoglossum laurae) is an Endangered species in Ohio where a single population is confined to a ~60 km segment of the Upper Mad River. Approximately 11,500 non-native brown trout (Salmo trutta) are stocked into the Upper Mad River annually to sustain a popular sport fishery. For this study, 27 brown trout were collected from the Upper Mad River. Individuals were euthanized on dry ice and transported to the laboratory where weight and total length were recorded. Total genomic DNA (gDNA) was extracted from the gut contents of each brown trout, and all gDNAs were amplified using the polymerase chain reaction (PCR) for a portion of the 16S ribosomal RNA (16S rRNA) gene. Next-generation sequencing of 16S rRNA amplicons identified several common fishes of the Upper Mad River. The gut contents of several brown trout also yielded the genetic signature of tonguetied minnow, a result that provides extremely strong support for predation by brown trout. Introductions of brown trout should be halted immediately to preserve Ohio's imperiled population of tonguetied minnow despite the high recreational and financial costs associated with this recommendation.

Tuesday January 29, 2019 6:00pm - 9:00pm
Superior Ballroom CD