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As a result of the prolonged government shutdown, we’re expecting some presentation cancellations and will continue to update the schedule with changes as they occur.  Otherwise the conference will proceed as planned.  Current cancellations and changes are listed here.

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Monday, January 28 • 1:40pm - 2:00pm
(SYMPOSIA-03) Environmental DNA Monitoring of Effectiveness of Bigheaded Carp Removal from Creve Coeur Lake, Missouri

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AUTHORS: Catherine A. Richter, Katy E. Klymus, Nathan Thompson, Jeffrey C. Jolley, Duane C. Chapman – U.S. Geological Survey

ABSTRACT: Creve Coeur Lake is a large natural floodplain lake intermittently connected to the Missouri River near St. Louis, Missouri. The lake has been invaded by Bighead Carp (Hypophthalmichthys nobilis) and Silver Carp (Hypophthalmichthys molitrix), collectively known as Bigheaded Carp. Both are native to Asia. The invasion has resulted in impairment of the native crappie (Pomoxis spp.) fishery, and hazards to recreational users. Fish can enter the lake from the Missouri River only during high water events. During the winter of 2017-2018, an intensive removal effort was conducted using the unified fishing method. A total of approximately 108,129 kg of Bigheaded Carp was removed from the lake in February 2018. Monitoring of Bigheaded Carp environmental DNA (eDNA) concentrations was conducted at intervals before and after the removal effort. Water was sampled at 53 locations equally spaced along transects covering the entire surface area of Creve Coeur Lake, a smaller upstream connected lake (Mallard Lake), and the channel between the two lakes. We measured eDNA concentrations with quantitative PCR using two marker sets specific to the genus Hypophthalmichthys, and thus able to detect and quantify DNA from both species with equal efficiency. Our results showed a decrease in eDNA concentration with decreasing water temperature over three sampling events before the removal effort, in October 2017, November 2017, and January 2018. After the removal effort, we observed an increase in eDNA in March 2018, possibly resulting from the presence of injured fish and carcasses, followed by a sharp decrease in eDNA in April 2018. Our results illustrate the utility of eDNA monitoring of management actions, the advantages of repeated sampling over time, and some challenges associated with this application of eDNA analysis.

Monday January 28, 2019 1:40pm - 2:00pm
HOPE BALLROOM C

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