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Welcome to the interactive web schedule for the 2019 Midwest Fish & Wildlife Conference! The schedule is subject to change (as of November 7, 2018). Please check back for updates. To return to the main Conference website, go to: www.midwestfw.org.

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Monday, January 28 • 1:20pm - 1:40pm
(SYMPOSIA-03) Targeting eDNA Surveys for the Invasive Black Carp, Mylopharyngodon piceus

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AUTHORS: Richard F Lance, Environmental Laboratory, US Army Engineer Research & Development Center; Xin Guan, Bennett Aerospace; Emy M. Monroe, Katherine D. Bockrath, Erica L. Mize – Whitney Genetics Laboratory, Midwest Fisheries Center, U.S. Fish and Wildlife Service; Chris B. Rees, Northeast Fishery Center, U.S. Fish and Wildlife Service; Kelly L. Baerwaldt, Midwest Fisheries Center, U.S. Fish and Wildlife Service

ABSTRACT: The Black Carp, Mylopharyngodon piceus, is an invasive species within the Mississippi River drainage that appears to be undergoing population growth and range expansion. Black carp are molluscivores that potentially threaten significant components of North America's rich indigenous diversity of freshwater bivalves. In order to help determine the presence of black carp in various waters and habitats, and to help track its spread, we have developed a suite of environmental DNA (eDNA) markers for this species. The markers were developed using whole mitochondrial genomes from 29 black carp from three countries and target three different mitochondrial DNA genes. The markers were further tested for reliability with a total of 41 black carp DNA samples and for specificity against DNA from numerous co-occurring fish species and against samples of natural waters free of black carp. Further tests to detect black carp in natural waters proved challenging, but ultimately successful. We further report on studies of which water fractions contain the bulk of black carp eDNA (the answer appears to be largely habitat dependent) and on the efficiency of different sampling options.

Monday January 28, 2019 1:20pm - 1:40pm
HOPE BALLROOM C