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Welcome to the interactive web schedule for the 2019 Midwest Fish & Wildlife Conference! The schedule is subject to change (as of November 20, 2018). Please check back for updates. To return to the main Conference website, go to: www.midwestfw.org.

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Monday, January 28 • 10:20am - 10:40am
(SYMPOSIA-03) A System for Rapid eDNA Detection of Aquatic Invasive Species

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AUTHORS: Austen Thomas, Smith-Root; Samantha Stanton, Michigan State University; Jake Ponce, Smith-Root; Mieke Sinnesael, Biomeme; Phong Nguyen, Smith-Root; Caren Goldberg, Washington State University

ABSTRACT: Environmental DNA (eDNA) detection of aquatic invasive species using PCR is a powerful new tool for resource managers, but laboratory results often take weeks to be produced which limits options for rapid management response. To circumvent laboratory delay, we combined a purpose-built eDNA filtration system (ANDe) with a field DNA extraction and handheld qPCR platform (Biomeme) to form a complete field eDNA sampling and detection process. A lab study involving serial dilution of New Zealand mudsnail eDNA was conducted to compare the detection capabilities of the field system with traditional bench qPCR. Two field validation studies were also conducted to determine if the on-site eDNA process can be used to map mudsnail eDNA distribution and quantify temporal fluctuations. Both platforms (Biomeme, bench qPCR) lost the ability to reliably detect mudsnail eDNA at the same dilution level (10<sup>-4</sup>), with SQ values as low as 21 DNA copies/reaction. A strong relationship was observed between the average Cq values of the two platforms (slope = 1.101, intercept = - 1.816, R<sup>2 </sup>= 0.997, P < 0.001). Of the 80 field samples collected, 44 (55%) tested positive for mudsnail eDNA with Biomeme, and results identified both spatial and temporal fluctuations in mudsnail eDNA/L. However, the PCR inhibition rate (no IPC amplification) with Biomeme was 28% on average for field samples, and up to 48% in the temporal dataset. With additional optimization of the DNA extraction process, the ANDe-Biomeme system has potential to be a rapid and highly effective detection/quantification tool for aquatic invasive species.

Monday January 28, 2019 10:20am - 10:40am